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1) DNA work:
a) Marathon-ReadyTM (RACE) - quick protocol and user manul.
b) Quickchange site-directed mutagenesis - user manual.
c) Gateway Vectors - TOPO vectors; pENTR vectors; pDONR vectors; pDEST vectors; pDEST 22 and 32 (2 hybrid); PWS4stopEGFP-A and pRc-CMV.
d) Novagen's LIC DuetTM adaptor kits - protocol.
e) Sequencing order sheet - here.
f) Restrictoin enzyme chart - link and Isoschizomers chart - link.
General Nucleic Acid Data - the chart *. DNA ladders - the picture.
2) Protein work:
a) ArcticExpress RIL and RP competent cells for low temprature expression-stratagene - Manual.
b) BL-21 Rosseta RARE competent cells (novagen) - theory.
c) Making mitotic lysate from Avram Hershko - protocol.
d) Coupling columns for antibody purification - protocol.
e) Purfication of GST-tagged proteins - protocol.
f) Immunodepletion of Hela extract from Avram - protocol.
g) FACS for mitotic profile - protocol.
Genaral Protein Data - the chart *. Protein ladders - the picture. Protein standard curve, Pierce - Calculate here.
3) Microscopy:
a) Important parameters of Yen lab's microscopes - the form.
b) Immunofluorescence staining - protocol.
c) Writing journals using Metamorph for Nikon TE2000 - protocol.
d) Preparing cells for EM from JB - protocol.
e) Improved Chromosome spread - protocol.
4) Recipes:
a) Proteinase and phosphotase inhibitors - recipe.
b) ATP regeneration system - recipe and theory.
c) Solutions for Immunofluorescence - recipe.
d) Making sodium phosphate solutions - recipe.
e) Preparation of PEI solution for transfection - recipe and protocol.
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